ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (10): 1697-1705.doi: 10.11843/j.issn.0366-6964.2015.10.001

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The Conception and Prospect of Detecting Phytase Activity by Immunological Techniques

HU Xiao-fei1,WEI Feng-xian2,YANG Ji-fei1,DENG Rui-guang1,ZHANG Gai-ping1,3*   

  1. (1.Henan Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Institute of Animal Husbandry and Veterinary Science,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;3.Henan Agricultural University,Zhengzhou 450002,China)
  • Received:2015-01-27 Online:2015-10-23 Published:2015-10-23

Abstract:

Phytic acid exists in food and feed widely,which can be combined with phosphorus and other nutrients to form insoluble complexes and subsequently reduce the phosphorus and other nutrient utilization rate.Phytase can decompose phytic acid and its salts to release of phosphorus and other nutrients,thus,phytase can be added in feed to replace part of dicalcium phosphate.However,if we reduce the amount of calcium hydrogen phosphate and forget to add phytase in feed in the same time,which will decline the feed quality,and subsequently lead to poor growth performance and production quality of animals.The measurement of phytase activity in feed is very important to ensure the quality of feed products.At present,the commonly detection methods are cumbersome and unsuitable for on-site detection of phytase activity for feed and animal husbandry enterprises.In this paper,a conception of detecting phytase activity with immunological techniques is proposed.Furthermore,we prospect the application of immunological techniques in the detection of phytase activity.

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